High-resolution infrared imaging of biological samples with third-order sum-frequency generation microscopy.

Publication Type:

Journal Article

Source:

Biomedical optics express, Volume 9, Issue 10, p.4807-4817 (2018)

URL:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179410/pdf/boe-9-10-4807.pdf

Abstract:

We studied the use of vibrationally resonant, third-order sum-frequency generation (TSFG) for imaging of biological samples. We found that laser-scanning TSFG provides vibrationally sensitive imaging capabilities of lipid droplets and structures in sectioned tissue samples. Although the contrast is based on the infrared-activity of molecular modes, TSFG images exhibit a high lateral resolution of 0.5 m or better. We observed that the imaging properties of TSFG resemble the imaging properties of coherent anti-Stokes Raman scattering (CARS) microscopy, offering a nonlinear infrared alternative to coherent Raman methods. TSFG microscopy holds promise as a high-resolution imaging technique in the fingerprint region where coherent Raman techniques often provide insufficient sensitivity.

Tags 
LAMMP
PMCID 
PMC6179410