Publication Type:Journal Article
Source:Applied optics, Volume 45, Issue 25, p.6421-8 (2006)
Keywords:Animals, Cell Movement, Cell Separation, Cells, Cultured, Dogs, Equipment Design, Equipment Failure Analysis, Lasers, Male, Micromanipulation, Spermatozoa
To study the chemotactic response of sperm to an egg and to characterize sperm motility, an annular laser trap based on axicons is designed, simulated with the ray-tracing tool, and implemented. The diameter of the trapping ring can be adjusted dynamically for a range of over 400 microm by simply translating one axicon along the optical axis. Trapping experiments with microspheres and dog sperm demonstrate the feasibility of the system, and the power requirement agrees with theoretical expectation. This new type of laser trapping could provide a prototype of a parallel, objective, and quantitative tool for animal fertility and biotropism study.