Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe.

Submitted by BLI_Admin on Thu, 07/28/2011 - 11:15

Publication Type:

Journal Article

Authors:

Liu, Gangjun; Kieu, Khanh; Wise, Frank W; Chen, Zhongping

Source:

Journal of biophotonics, Volume 4, Issue 1-2, p.34-9 (2011)

Keywords:

Animals, Fluorescent Dyes, Lasers, Microscopy, Molecular Imaging, Photons, Rats, Tendons, Time Factors

Abstract:

We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.

PDF File:

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Tags:

LAMMP