Integrated Optical Systems for Laser Nanosurgery and Optical Trapping to Study Cell Structure and Function

Publication Type:

Book Chapter


Current Microscopy Contributions to Advances in Science and Technology (2012)


There is an increasing interest in the use of laser micromanipulation tools to study repair mechanisms, motility,
biomechanics, and physiology at the cellular and subcellular levels. We describe laser scissors and trapping systems that
allow use of either of these optical tools alone, or in combination with each other. For micro or nano-ablation the laser
wavelength may vary from UV, green, near IR to IR. The pulse duration of the laser may be in the nanosecond,
picosecond, or femtosecond regimes. The parameters used will depend upon such factors as, desired spot size, absorption
properties (natural or applied), and ablation mechanisms (single photon or multiphoton). We will review several biological
problems where different laser parameters are used for ablation as well as studies where optical trapping is used alone, and
preliminary studies where both trapping and ablation are used together in the same cell.

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