Vibrationally resonant sum-frequency generation microscopy with a solid immersion lens.

Publication Type:

Journal Article

Source:

Biomedical optics express, Volume 5, Issue 7, p.2125-34 (2014)

Abstract:

We use a hemispheric sapphire lens in combination with an off-axis parabolic mirror to demonstrate high-resolution vibrationally resonant sum-frequency generation (VR-SFG) microscopy in the mid-infrared range. With the sapphire lens as an immersed solid medium, the numerical aperture (NA) of the parabolic mirror objective is enhanced by a factor of 1.72, from 0.42 to 0.72, close to the theoretical value of 1.76 ( = nsapphire). The measured lateral resolution is as high as 0.64 μm. We show the practical utility of the sapphire immersion lens by imaging collagen-rich tissues with and without the solid immersion lens.

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PMCID 
PMC4102353