@article {1055,
title = {Hyperspectral optical tomography of intrinsic signals in the rat cortex.},
journal = {Neurophotonics},
volume = {2},
year = {2015},
month = {2015 Oct},
pages = {045003},
abstract = {We introduce a tomographic approach for three-dimensional imaging of evoked hemodynamic activity, using broadband illumination and diffuse optical tomography (DOT) image reconstruction. Changes in diffuse reflectance in the rat somatosensory cortex due to stimulation of a single whisker were imaged at a frame rate of 5~Hz using a hyperspectral image mapping spectrometer. In each frame, images in 38 wavelength bands from 484 to 652~nm were acquired simultaneously. For data analysis, we developed a hyperspectral DOT algorithm that used the Rytov approximation to quantify changes in tissue concentration of oxyhemoglobin ([Formula: see text]) and deoxyhemoglobin (ctHb) in three dimensions. Using this algorithm, the maximum changes in [Formula: see text] and ctHb were found to occur at [Formula: see text] and [Formula: see text] beneath the surface of the cortex, respectively. Rytov tomographic reconstructions revealed maximal spatially localized increases and decreases in [Formula: see text] and ctHb of [Formula: see text] and [Formula: see text], respectively, with these maximum changes occurring at [Formula: see text] poststimulus. The localized optical signals from the Rytov approximation were greater than those from modified Beer-Lambert, likely due in part to the inability of planar reflectance to account for partial volume effects.},
issn = {2329-423X},
doi = {10.1117/1.NPh.2.4.045003},
author = {Soren D. Konecky and Wilson, Robert H and Hagen, Nathan and Mazhar, Amaan and Tkaczyk, Tomasz S and Frostig, Ron D and Tromberg, Bruce J}
}