TY - JOUR
T1 - Hyperspectral optical tomography of intrinsic signals in the rat cortex.
JF - Neurophotonics
Y1 - 2015
A1 - Soren D. Konecky
A1 - Wilson, Robert H
A1 - Hagen, Nathan
A1 - Mazhar, Amaan
A1 - Tkaczyk, Tomasz S
A1 - Frostig, Ron D
A1 - Tromberg, Bruce J
AB - We introduce a tomographic approach for three-dimensional imaging of evoked hemodynamic activity, using broadband illumination and diffuse optical tomography (DOT) image reconstruction. Changes in diffuse reflectance in the rat somatosensory cortex due to stimulation of a single whisker were imaged at a frame rate of 5 Hz using a hyperspectral image mapping spectrometer. In each frame, images in 38 wavelength bands from 484 to 652 nm were acquired simultaneously. For data analysis, we developed a hyperspectral DOT algorithm that used the Rytov approximation to quantify changes in tissue concentration of oxyhemoglobin ([Formula: see text]) and deoxyhemoglobin (ctHb) in three dimensions. Using this algorithm, the maximum changes in [Formula: see text] and ctHb were found to occur at [Formula: see text] and [Formula: see text] beneath the surface of the cortex, respectively. Rytov tomographic reconstructions revealed maximal spatially localized increases and decreases in [Formula: see text] and ctHb of [Formula: see text] and [Formula: see text], respectively, with these maximum changes occurring at [Formula: see text] poststimulus. The localized optical signals from the Rytov approximation were greater than those from modified Beer-Lambert, likely due in part to the inability of planar reflectance to account for partial volume effects.
VL - 2
IS - 4
U1 - http://www.ncbi.nlm.nih.gov/pubmed/26835483?dopt=Abstract
ER -